murine calvaria-derived preosteoblast mc3t3e1 cell line  (Procell Inc)

Journal: RSC Advances

Article Title: Fabrication and in vitro cytocompatibility evaluation of porous bone scaffold based on cuttlefish bone-derived nano-carbonated hydroxyapatite reinforced with polyethylene oxide/chitosan fibrous structure

doi: 10.1039/d4ra08457h

Figure Lengend Snippet: (a) Viabilities of MC3T3E1 cells, (b) IC 50 analysis by non-linear curve fitting, and (c) IC 50 values of the nCHA/PEO/CS-21 and nCHA/PEO/CS-11 scaffolds. *** p < 0.001.

Article Snippet: MC3T3E1 cells (European Collection of Authenticated Cell Cultures (ECACC) No. 99072810, UK) were cultured in MEM-α medium (Gibco, USA) with 10% fetal bovine serum (FBS, Gibco, CA, USA), 2% penicillin–streptomycin (Gibco, CA, USA), and 0.5% fungizone (Gibco, USA).

Techniques:

Journal: RSC Advances

Article Title: Fabrication and in vitro cytocompatibility evaluation of porous bone scaffold based on cuttlefish bone-derived nano-carbonated hydroxyapatite reinforced with polyethylene oxide/chitosan fibrous structure

doi: 10.1039/d4ra08457h

Figure Lengend Snippet: Average cell viabilities of MC3T3E1 cells against the nCHA/PEO/CS-21 and nCHA/PEO/CS-11 scaffolds

Article Snippet: MC3T3E1 cells (European Collection of Authenticated Cell Cultures (ECACC) No. 99072810, UK) were cultured in MEM-α medium (Gibco, USA) with 10% fetal bovine serum (FBS, Gibco, CA, USA), 2% penicillin–streptomycin (Gibco, CA, USA), and 0.5% fungizone (Gibco, USA).

Techniques: Concentration Assay

Journal: RSC Advances

Article Title: Fabrication and in vitro cytocompatibility evaluation of porous bone scaffold based on cuttlefish bone-derived nano-carbonated hydroxyapatite reinforced with polyethylene oxide/chitosan fibrous structure

doi: 10.1039/d4ra08457h

Figure Lengend Snippet: Morphology of MC3T3E1 cells after 24 incubation in conditioned medium with serial concentrations of (a–d) nCHA/PEO/CS-21 and (e–h) nCHA/PEO/CS-11 scaffolds. The white dashed circle, orange, purple, and blue arrowheads indicate cell cluster, life cells, dead cells, and scaffold particles, respectively. Scale bars: 100 μm (a–h).

Article Snippet: MC3T3E1 cells (European Collection of Authenticated Cell Cultures (ECACC) No. 99072810, UK) were cultured in MEM-α medium (Gibco, USA) with 10% fetal bovine serum (FBS, Gibco, CA, USA), 2% penicillin–streptomycin (Gibco, CA, USA), and 0.5% fungizone (Gibco, USA).

Techniques: Incubation